Oral presentation 2011 Focus Meeting available

Slide decks of the of the oral presentations of the 2011 EBF Focus Meeting "Large meets Small" have been made available for public viewing and or download at the conference site:

http://bru2011.europeanbioanalysisforum.eu/slides

Photos of the 2011 EBF Focus Meeting "Large meets Small"

The second Focus Meeting organised by the European Bioanalysis Forum attracted a good international fearless*) crowd of a bit over 180 scientists from academia, industry and contract service organisations.

A conference report is being prepared by the Organising Committee (to be published in Bioanalysis) and soon the slide decks will be made available on the meeting website.

In the mean time you can already view and/or download the pictures by visiting either the EBF Facebook page or our Picasa album.

*) see: http://ebfnewsletter.blogspot.com/2011/05/are-bioanalysts-afraid-of-grimsvotn.html

EBF membership open to CROs

As presented during the 3rd Open Symposium in Barcelona in 2010, EBF has changed its structure and has slightly adapted her groundrules to facilitate CROs becoming a member of the European Bioanalysis Forum. CROs being interested to become a member of EBF are advised to view the attached slide deck:

Donwload the EBF-CRO integration slide deck (pdf)

and submit an EBF member company application form.

Are Bioanalysts afraid of Grimsvotn?

Only 4 weeks left before the “Large meets Small” focus meeting organized by the European Bioanalysis Forum kicks off and we have a volcanic eruption in Iceland again. Not Eyjafjallajokull, but Grimsvotn this time. Did the first one ruin the travelling home of many of our colleagues after the Dried Blood Spots focus meeting, this one seems to behave better. At least it erupts well before the meeting and not on the 22nd of June!

Do not forget to register in time for this landmark meeting in which bioanalytical scientists will connect on strategies on analyzing large molecules with small molecule technologies

The 2 day program comprises 6 sessions ranging from New Instrument Developments and Cutting Edge Analytical Approaches to Proteins in the MIST. Download or view the conference agenda of this exciting meeting on the EBF Focus Meeting website

Register now using the designated form to save € 150 on the on-site registration fee.

e-Registration: € 250             On-site Registration: € 400

Looking forward to see you in Brussels, Belgium - 21st to 22nd of June 2011 !


EBF Focus Meeting Organization Committee:
Peter van Amsterdam, Philip Timmerman, Silke Luedtke, Berthold Lausecker & Begona Barroso

EBF 4th Open Symposium - Call for Speakers - Call for Posters

1st Call for Speakers

European Bioanalysis Forum

4th Open Symposium

Less is More

16-18 November 2011
Hesperia Tower Hotel

Barcelona • Spain

INTRODUCTION

Over 400 participants contribute to the success of the EBF Open Symposia in Barcelona. Building further on this event, EBF announces the 4th Open Symposium. The meeting will be held in Barcelona at the Hesperia Tower Conference Center from the 16th – 18th November 2011.

With the theme of the 4th open Symposium being "Less is More", EBF wants to focus on new trends in Bioanalysis in which scientists are eager to get more value from an optimized regulatory environment (where global harmonization can bring relief by decreasing the number of different guidelines) and from new technology developments (allowing miniaturization and increased sensitivity). Again, we will be bringing a versatile and diverse program that combines regulated bioanalysis themes (including updates on EMA guidelines and GBC progress) with technological and scientific developments for small and large molecule bioanalysis.

MEETING SESSIONS will include

• Biomarker validation recommendation
• Effect of hemolysed / lipidemic / ”off target” matrices on bioanalytical assays
• Regulations and Bioanalysis - update on new and emerging guidelines and on Global harmonization
• Technology updates
• The relationship between Incurred Sample Reanalysis and Incurred Sample Stability
• Micro dosing versus micro tracer – AMS or sensitive traditional analysis
• Challenges of "free" and "total" macromolecule quantification
• Dried Blood Spots - updates on blood analysis
• Further focus on LBA - topic to be announced
• Anomalous results

For details on the goals and objectives of each session, please visit the conference website at http://bcn2011.europeanbioanalysisforum.eu/

CALL FOR SPEAKERS

Potential speakers, please submit the title and abstract of your presentation (before 15 September 2011) to conference address: bcn2011@europeanbioanalysisforum.eu including a completed oral presentation submission form available on the conference website. Acceptance of your presentation will be communicated before 30 September 2011.

CALL FOR POSTERS

Please submit the title and abstract of your poster (before 15 October 2011) to conference address: bcn2011@europeanbioanalysisforum.eu including a completed poster presentation submission form available on the conference website. Supported Poster format is Portrait (size A0).

2011 Open EBF Symposium Website Live!

Over 400 participants contribute to the success of the EBF Open Symposia in Barcelona. Building further on this event, EBF announces the 4th Open Symposium. The meeting will be held in Barcelona at the Hesperia Tower Conference Center from the 16th - 18th November 2011. With the theme of the 4th open Symposium being "Less is More", EBF wants to focus on new trends in Bioanalysis in which scientists are eager to get more value from an optimized regulatory environment (where global harmonization can bring relief by decreasing the number of different guidelines) and from new technology developments (allowing miniaturization and increased sensitivity). Again, we will be bringing a versatile and diverse program that combines regulated bioanalysis themes (including updates on EMA guidelines and GBC progress) with technological and scientific developments for small and large molecule bioanalysis.

MEETING SESSIONS will include

• Biomarker validation recommendation
• Effect of haemolysed / lipidemic / "off target" matrices on bioanalytical assays
• Regulations and Bioanalysis - update on new and emerging guidelines and on Global harmonization
• Technology updates
• The relationship between Incurred Sample Reanalysis and Incurred Sample Stability
• Micro dosing versus micro tracer - AMS or sensitive traditional analysis
• Challenges of "free" and "total" macromolecule quantification
• Dried Blood Spots - updates on blood analysis
• Further focus on LBA - topic to be announced
• Anomalous results

Visit the Open Symposium website at: http://bcn2011.europeanbioanalysisforum.eu/

Register now for the EBF June 2011 Focus Meeting

Only 5 weeks left before the “Large meets Small” focus meeting organized by the European Bioanalysis Forum kicks off. Do not forget to register in time for this landmark meeting in which bioanalytical scientists will connect on strategies on analyzing large molecules with small molecule technologies

The 2 day program comprises 6 sessions ranging from New Instrument Developments and Cutting Edge Analytical Approaches to Proteins in the MIST. Download or view the conference agenda of this exiting meeting on the EBF Focus Meeting website

Register now using the designated form to save € 150 on the on-site registration fee.

e-Registration: € 250             On-site Registration: € 400

Looking forward to see you in Brussels, Belgium - 21st to 22nd of June 2011 !


EBF Focus Meeting Organization Committee:
Begona Barroso, Berthold Lausecker, Peter van Amsterdam, Philip Timmerman & Silke Luedtke

Update on GBC: All Harmonsation Team leads have been assigned

All Molecules / All Techniques

A1 - Scope and Regulations : Surendra Bansal
A2 - Tiered approaches for method validation : Steve Lowes
A3 - Method transfer, partial/cross validations : Ray Briggs
A4 - Reference standards and reagents : Joseph Bower
A5 - Sample Management : Mike Redrup
A6 - Stability : Nico van de Merbel
A7 - Repeat analysis and ISR : Eric Fluhler
A8 - Documentation : Tom Verhaeghe
A9 - Analytical instrument qualification : Chad Briscoe
A10 - New Frontiers : Robert Benthem
A11 - Biomarkers : Howard Hill

Large Molecules / Ligand Binding

L1 - Large molecule specific run acceptance : Marian Kelley
L2 - Large molecule specific assay operation : Lauren Stevenson
L3 - Assay formats : Sherri Dudal
L4 - Reagents and their stability : Lindsay King
L5 - Automation practices in LM bioanalysis : Han Gunn
L6 - Immunogenicity - Effect on PK : Jeff Sailstad

Small Molecules / Chromatographic Assays

S1 - Small molecule specific run acceptance : Doug Fast
S2 - Small molecule specific assay operation : Eric Woolf
S3 - Chromatographic run quality assessment : Stuart McDougall

Agenda EBF 2011 Focus Meeting - Large meets Small

Day 0 – 20 JUNE 2011

Registration and Information Desk Open @4:00 pm

Day 1 - 21 JUNE 2011

Registration and Information Desk Open @7:30 am

New Technology Developments for Analysis of Large Molecules

Comparison of different MS platforms for peptide quantification
Lieve Dillen, Janssen R&D, Belgium

Implementation of a common high pH LC/MS/MS platform for small molecules and large molecules
Robert Plumb, Waters Corporation

Introducing a novel bench top Orbitrap for highly selective peptide/protein quantitation
Kai Scheffler, Thermo Fisher Scientific

High Sensitivity Protein Quantitation Using a Triple Quadrupole with a Dual Ion Funnel
Lester Taylor, Agilent Technologies

Coffee Break

Cutting Edge Analytical Approaches - Peptides

Strategies for the analysis of therapeutic peptides in biofluids
Lee Goodwin, Covance

Comparison of clinical analyser and LC-MS/MS based approaches for quantification of serum IGF-I levels
Rachel Green, Quotient Bioresearch

Quantitative LC-MS/MS analysis of polypeptides: glucagon
Veniamin Lapko, Celerion

Quantification of selected peptides in biological fluids e.g. plasma
Hermann Mascher, pharm-analyt Lab. GmbH

Lunch

Cutting Edge Analytical Approaches - Peptides

The importance of sample preparation for the analysis of proteins and peptides by liquid chromatography with mass spectrometric detection
Gerard Hopfgartner, University of Geneva

LC-MS/MS bioanalysis of therapeutic peptides in plasma at low pg/mL levels
Magnus Knutsson, Ferring Pharmaceuticals A/S

A selective SPE/LC-MS/MS assay for simultaneous quantitation of multiple amyloid beta peptides in cerebrospinal fluid
Erin Chambers, Waters Corporation

Coffee Break

Validation Requirements for Large Molecule Analysis with MS Techniques

EBF presentation

Validation of an immunoprecipitation, digestion and immunoaffinity LC-MS/MS assay for human NGF biomarker and implementation in support of clinical trials
Gary Schulz, Advion Bioservices

The use of internal standards for macromolecule quantification by LC-MS: lessons learned from small molecule assays
Nico van de Merbel, PRA International

Issues of specificity, recovery, and accuracy in LC-MS/MS assays for peptides and proteins (working title)
Ben van Baar, QPS

Panel Discussion

Cocktail Reception


Day 2 - 22 JUNE 2011

Cutting Edge Analytical Approaches - Proteins

The powers of using MRM3 and high resolution MS with fast scan rates to conquer previously difficult large molecule compounds
Yong-Xi Li, Medpace Bioanalytical Laboratories

LC-MS/MS quantification of proteins and peptides in biological fluids: towards a generic approach
Olivier Heudi, Novartis Pharma AG

High throughput method for the quantitative analysis of therapeutic antibody in the low ng/mL range from serum samples via affinity enrichment coupled LC-MS/MS to support clinical and preclinical PK/TK evaluation
Stephanie Fischmann, Abbott

Coffee Break

Sample preparation for protein quantification by LC-MS/MS
Ejvind Mørtz, Alphalyse

Challenges in validating protein and peptide LC-MS/MS methodologies
Ian Ward, Quotient Bioresearch

Comparison of quantitative assays for a large protein: ELISA vs. LC-MS/MS
Alberto Guenzi, Hoffmann-La Roche

Development and validation of an immunoaffinity-LC-MS/MS assay for 22 KDa somatropin in human serum (working title)
Rand Jenkins, PPD

Lunch

Analytical Strategies

The sense and nonsense of quantitative analysis of biologics - or why should we care
Martin Nemansky, PRA International

Ligand Binding Assay critical reagent characterization via LC-MS
Brian Geist, Centocor

Highly sensitive Cytokine Quantification using Imperacer®, an Immuno-PCR based Ligand-Binding Assay System
Michael Adler, Chimera Biotec GmbH

Coffee Break

Proteins in the MIST

Ion mobility mass spectrometry: New molecular tool for the analysis of reactive intermediates in the oligomerization- aggregation of “misfolding”-aggregating proteins
Michael Przybylski, University of Konstanz

Strategies for quantifying plasma protease susceptible peptides by LC-MS/MS
Richard Kay, Quotient Bioresearch

Bioanalytical LC-MS of therapeutic oligonucleotides
William van Dongen, PROXY laboratories

Extracting metabolite ions of peptide drugs out of an in vivo matrix background
Filip Cuyckens, Janssen R&D

Closing Remarks and Adjourn

EBF 2011 Focus Meeting: Last Call for Posters!

Their is still space for a couple of more scientific posters and the EBF Organisation Committee has thus decided to extend the poster submission deadline to 15 May 2011. So there is still time to submit one or more poster presentations for the EBF "Large meets Small" Focus Meeting

Poster Submission:

http://bru2011.europeanbioanalysisforum.eu/posters

Meeting Agenda:

http://bru2011.europeanbioanalysisforum.eu/agenda

Registration fees 2011 European Bioanalysis Forum Focus Meeting

• e-Registration: € 250
• On-site Registration: € 400

http://bru2011.europeanbioanalysisforum.eu/registration

Join us in Brussels, Belgium - 21st to 22nd of June 2011 !